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Article

A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa.

Molecular and cellular probes - 1 Aug 2016

Boubaker Ghalia, Marinova Irina, Gori Francesca, Hizem Amani, Müller Norbert, Casulli Adriano, Jerez Puebla Luis Enrique, Babba Hamouda, Gottstein Bruno, Spiliotis Markus

Abstract excerpt

Reliable and rapid molecular tools for the genetic identification and differentiation of Echinococcus species and/or genotypes are crucial for studying spatial and temporal transmission dynamics. Here, we describe a novel dual PCR targeting regions in the small (rrnS) and large (rrnL) subunits of mitochondrial ribosomal RNA (rRNA) genes, which enables (i) the specific identification of species and genotypes of...

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PMID: 27242008
DOI: 10.1016/j.mcp.2016.05.004

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