Article
Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues.
International journal for parasitology - 1 Jul 2008
Schneider Renate, Gollackner Bernd, Edel Bernhard, Schmid Katharina, Wrba Friedrich, Tucek Georg, Walochnik Julia, Auer Herbert
Abstract excerpt
The aim of our study was to establish a new PCR protocol for the detection and discrimination of Echinococcus granulosus complex on one hand and Echinococcus multilocularis in formalin-fixed, paraffin-embedded tissues (FFPTs) on the other. The target sequences for all PCRs are located on a 471bp segment of the mitochondrial ND1 gene, the fragment sizes of the amplification products are 295bp (for the sheep strain...
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