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Multiplex PCR-based RFLP assay for early identification of prevalent Mycobacterium leprae genotypes.

Diagnostic microbiology and infectious disease - 1 Dec 2023

Dwivedi Purna, Sharma Mukul, Singh Pushpendra

Abstract excerpt

Mycobacterium leprae is classified into four SNP genotypes and 16 subtypes (from 1A to 4P) that exhibit phylogeographical association reported from around the world. Among them, genotypes 1D and 3I represent more than 60% of M. leprae strains. Here, we report a new method for M. leprae genotyping which identifies the genotypes 1D and 3I by combining multiplex PCR amplification and restriction fragment length...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 37832201
DOI: 10.1016/j.diagmicrobio.2023.116084

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