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Impact of nanopore-based metagenome sequencing on tick-borne virus detection

30 May 2023

Abstract excerpt

Introduction: We evaluated metagenomic nanopore sequencing (NS) in field-collected ticks and compared findings from amplification-based assays. Methods: Forty tick pools collected in Anatolia, Turkey and screened by broad-range or nested polymerase chain reaction (PCR) for Crimean-Congo Hemorrhagic Fever Virus (CCHFV) and Jingmen tick virus (JMTV) were subjected to NS using a standard, cDNA-based metagenome...

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PMID: 37323891
DOI: 10.3389/fmicb.2023.1177651

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