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Article

[Rapid detection and genotyping of SARS-CoV-2 Omicron BA.4/5 variants using a RT-PCR and CRISPR-Cas12a-based assay].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 20 Apr 2023

Ma Y, Zou L, Liang Y, Liu Q, Sun Q, Pang Y, Lin H, Deng X, Tang S

Abstract excerpt

OBJECTIVE: To establish a rapid detection and genotyping method for SARS-CoV-2 Omicron BA.4/5 variants using CRISPPR-Cas12a gene editing technology. METHODS: We combined reverse transcription-polymerase chain reaction (RT-PCR) and CRISPR gene editing technology and designed a specific CRISPPR RNA (crRNA) with suboptimal protospacer adjacent motifs (PAM) for rapid detection and genotyping of SARS- CoV-2 Omicron...

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Source

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Identifiers

PMID: 37202186
DOI: 10.12122/j.issn.1673-4254.2023.04.03
PMCID: PMC10202775

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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