Article
Multiple mutations of Mycobacterium intracellulare subsp. chimaera causing false-negative reaction to the transcription-reverse transcription concerted method for pathogen detection.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Aug 2023
Togawa Atsushi, Chikamatsu Kinuyo, Takaki Akiko, Matsumoto Yuki, Yoshimura Michinobu, Tsuchiya Shigeo, Nakamura Shota, Mitarai Satoshi
Abstract excerpt
OBJECTIVES: To report an isolate of Mycobacterium intracellulare subsp. chimaera with multiple mutations in 16S ribosomal RNA (rRNA) gene, resulting in the false-negative reaction to the transcription-reverse transcription concerted (TRC) method for Mycobacterium avium-intracellulare complex. METHODS: We used TRC, polymerase chain reaction (PCR), and Matrix-assisted laser desorption/ionization Time-of-Flight/Mass...
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