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Article

Rapid Generation of Barley Homozygous Transgenic Lines Based on Microspore Culture: HvPR1 Overexpression as an Example.

International journal of molecular sciences - 3 Mar 2023

Chen Zhiwei, Jiang Qi, Guo Guimei, Shen Qiufang, Yang Jun, Wang Ertao, Zhang Guoping, Lu Ruiju, Liu Chenghong

Abstract excerpt

Obtaining homozygous lines from transgenic plants is an important step for phenotypic evaluations, but the selection of homozygous plants is time-consuming and laborious. The process would be significantly shortened if anther or microspore culture could be completed in one generation. In this study, we obtained 24 homozygous doubled haploid (DH) transgenic plants entirely by microspore culture from one T0...

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Identifiers

PMID: 36902374
DOI: 10.3390/ijms24054945
PMCID: PMC10003194

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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