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Development and validation of a one-tube, nested real-time PCR method suitable for routine detection ofMycobacterium bovisin animal tissue

23 Feb 2023

Abstract excerpt

AIMS: Development and validation of a real-time PCR test for high-throughput routine screening of animal tissue for Mycobacterium bovis and other Mycobacterium tuberculosis complex (MTBC) members. METHODS AND RESULTS: A preliminary study compared the results of a combination of five tissue preparation/DNA extraction methods and nine PCR assays on a panel of 92 cattle tissue samples of known M. bovis culture...

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PMID: 36822626
DOI: 10.1093/jambio/lxad038

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