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Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

11 Mar 2014

Abstract excerpt

In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA...

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PMID: 24618787
DOI: 10.1371/journal.pone.0091023

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