Article
Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus.
Journal of applied microbiology - 1 Mar 2023
Bertolini Amanda Bezerra, Thyssen Patrícia Jacqueline, Brandão Paulo Eduardo, Prado Aline Marrara, Silva Sheila Oliveira de Souza, Mioni Mateus de Souza Ribeiro, de Gouvea Fábio Lucas Rezende, Pantoja José Carlos de Figueiredo, Langoni Helio, Guimarães Felipe Freitas, Joaquim Sâmea Fernandes, Guerra Simony Trevizan, Leite Domingos da Silva, Rall Vera Mores, Hernandes Rodrigo Tavanelli, Lucheis Simone Baldini, Rossi Gabriel Augusto Marques, Ribeiro Márcio Garcia
Abstract excerpt
AIMS: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. METHODS AND RESULTS: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae...
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