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Article

Using CRISPR/Cas9 to generate a heterozygous COL2A1 p.R719C iPSC line (MCRIi019-A-6) model of human precocious osteoarthritis.

Stem cell research - 1 Mar 2023

Yammine Kathryn M, Mirda Abularach Sophia, Sampurno Lisa, Bateman John F, Lamandé Shireen R, Shoulders Matthew D

Abstract excerpt

The human iPSC line MCRIi019-A-6 was generated using CRISPR/Cas9-mediated gene editing to introduce a heterozygous COL2A1 exon 33 c.2155C>T (p.R719C) mutation into the control human iPSC line MCRIi019-A. Both the edited and parental lines display typical iPSC characteristics, including the expression of pluripotency markers, the ability to be differentiated into the three germ lines, and a normal karyotype. This...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 36682125
DOI: 10.1016/j.scr.2023.103020
PMCID: PMC10038005

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