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An updated protocol for in vitro bovine embryo production

13 Dec 2022

Abstract excerpt

Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A...

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PMID: 36520625
DOI: 10.1016/j.xpro.2022.101924

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