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Article

Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype.

PloS one - 1 Jan 2022

Bond J J, Donaldson A J, Woodgate S, Kamath K S, Mckay M J, Wheeler D, Tucker D, Oddy V H

Abstract excerpt

BACKGROUND: Ruminant livestock are a major contributor to Australian agricultural sector carbon emissions. Variation in methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen of sheep differs with different digestive functions. METHOD: We isolated rumen epithelium enzymatically to extract membrane and cytosol proteins from sheep with high (H) and low (L) CH4 emission. Protein...

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Carbon
Fermentation
Lactates
Methane
Pyruvaldehyde

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PMID: 36256644
DOI: 10.1371/journal.pone.0273184
PMCID: PMC9578583

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