Targeted full-genome amplification and sequencing of dengue virus types 1-4 from South America.

doi:10.1016/j.jviromet.2016.06.001

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Article

Targeted full-genome amplification and sequencing of dengue virus types 1-4 from South America.

Journal of virological methods - 1 Sept 2016

Cruz Cristopher D, Torre Armando, Troncos Gilda, Lambrechts Louis, Leguia Mariana

Abstract excerpt

We report optimized workflows for full-genome sequencing of dengue viruses (DENVs) 1-4. Based on alignments of publicly available complete genomes we modified and expanded existing primers sets to amplify DENV genotypes that were previously difficult or impossible to sequence. We also report improvements to streamline laboratory handling, including a dual amplification strategy for easy and difficult to sequence...

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PMID: 27334982
DOI: 10.1016/j.jviromet.2016.06.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.