Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations.

doi:10.1016/0166-0934(92)90165-a

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Article

Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations.

Journal of virological methods - 1 Jul 1992

Lewis J G, Chang G J, Lanciotti R S, Trent D W

Abstract excerpt

The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific prim...

Topics

Base Sequence
DNA, Viral
Dengue Virus
Epidemiologic Methods
Genetic Variation
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Viral
RNA-Directed DNA Polymerase

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