Article
Improved Growth and High Inheritance of Melanocortin-4 Receptor (mc4r) Mutation in CRISPR/Cas-9 Gene-Edited Channel Catfish, Ictalurus punctatus.
Marine biotechnology (New York, N.Y.) - 1 Oct 2022
Coogan Michael, Alston Veronica, Su Baofeng, Khalil Karim, Elaswad Ahmed, Khan Mohd, Johnson Andrew, Xing De, Li Shangjia, Wang Jinhai, Simora Rhoda M C, Lu Cuiyu, Page-McCaw Patrick, Chen Wenbiao, Michel Max, Wang Wenwen, Hettiarachchi Darshika, Hasin Tasnuba, Butts Ian A E, Cone Roger D, Dunham Rex A
Abstract excerpt
Effects of CRISPR/Cas9 knockout of the melanocortin-4 receptor (mc4r) gene in channel catfish, Ictalurus punctatus, were investigated. Three sgRNAs targeting the channel catfish mc4r gene in conjunction with Cas9 protein were microinjected in embryos and mutation rate, inheritance, and growth were studied. Efficient mutagenesis was achieved as demonstrated by PCR, Surveyor® assay, and DNA sequencing. An overall...
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