Article
CD4 bovine gene: Differential polymorphisms among cattle breeds and a new tool for rapid identification.
Veterinary immunology and immunopathology - 1 Sept 2022
Okino Cintia Hiromi, Malagó Junior Wilson, Marcondes Cintia Righetti, Giglioti Rodrigo, Montassier Hélio José, Oliveira Henrique Nunes de, Oliveira Márcia Cristina de Sena
Abstract excerpt
Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and...
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