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Article

CRISPR-Cas9-mediated mutagenesis of kiwifruit BFT genes results in an evergrowing but not early flowering phenotype.

Plant biotechnology journal - 1 Nov 2022

Herath Dinum, Voogd Charlotte, Mayo-Smith Matthew, Yang Bo, Allan Andrew C, Putterill Joanna, Varkonyi-Gasic Erika

Abstract excerpt

Phosphatidylethanolamine-binding protein (PEBP) genes regulate flowering and architecture in many plant species. Here, we study kiwifruit (Actinidia chinensis, Ac) PEBP genes with homology to BROTHER OF FT AND TFL1 (BFT). CRISPR-Cas9 was used to target AcBFT genes in wild-type and fast-flowering kiwifruit backgrounds. The editing construct was designed to preferentially target AcBFT2, whose expression is elevated...

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PMID: 35796629
DOI: 10.1111/pbi.13888
PMCID: PMC9616528

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