Article
Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] - 1 Sept 2022
Gularte Juliana Schons, da Silva Mariana Soares, Filippi Micheli, Demoliner Meriane, Schallenberger Karoline, Hansen Alana Witt, de Abreu Góes Pereira Vyctoria Malayhka, Heldt Fágner Henrique, Girardi Viviane, Weber Matheus Nunes, de Almeida Paula Rodrigues, Abbadi Bruno Lopes, Dornelles Maiele, Bizarro Cristiano Valim, Machado Pablo, Basso Luiz Augusto, Dellagostin Odir Antonio, Fleck Juliane Deise, Spilki Fernando Rosado
Abstract excerpt
Different approaches are in use to improve our knowledge about the causative agent of coronavirus disease (COVID-19). Cell culture-based methods are the better way to perform viral isolation, evaluate viral infectivity, and amplify the virus. Furthermore, next-generation sequencing (NGS) have been essential to analyze a complete genome and to describe new viral species and lineages that have arisen over time....
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