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Article

A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species.

PloS one - 4 May 2011

Elshire Robert J, Glaubitz Jeffrey C, Sun Qi, Poland Jesse A, Kawamoto Ken, Buckler Edward S, Mitchell Sharon E

Abstract excerpt

Advances in next generation technologies have driven the costs of DNA sequencing down to the point that genotyping-by-sequencing (GBS) is now feasible for high diversity, large genome species. Here, we report a procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs). This approach is simple, quick, extremely specific, highly reproducible, and may reach important...

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Identifiers

PMID: 21573248
DOI: 10.1371/journal.pone.0019379
PMCID: PMC3087801

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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