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Exercising the Sanger Sequencing Strategy for Variants Screening and Full-Length Genome of SARS-CoV-2 Virus during Alpha, Delta, and Omicron Outbreaks in Hiroshima.

Viruses - 30 Mar 2022

Ko Ko, Takahashi Kazuaki, Nagashima Shintaro, E Bunthen, Ouoba Serge, Takafuta Toshiro, Fujii Yoshiki, Mimori Michi, Okada Fumie, Kishita Eisaku, Ariyoshi Kunie, Hussain Md Razeen Ashraf, Sugiyama Aya, Akita Tomoyuki, Kuwabara Masao, Tanaka Junko

Abstract excerpt

This study aimed to exercise the Sanger sequencing strategy for screening of variants among confirmed COVID-19 cases and validate our strategy against NGS strains in Hiroshima retrieved from GISAID. A total of 660 samples from confirmed COVID-19 cases underwent screening for variants by Sanger-based partial sequencing to the targeted spike gene (nt22,735~nt23,532) using an in-house-developed primer set. The...

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PMID: 35458450
DOI: 10.3390/v14040720
PMCID: PMC9030034

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