Article
Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing.
Journal of virological methods - 1 May 2022
Xiao Yihan, Ni Lan, Cui Zhigang, Sun Long, Zhou Xiaojun, Chen Xinjun, Li Lihua, Tang Chuanning, Yin Feifei, Du Jiang, Niu Lina, Lu Gang, Cui Xiuji, Quan Yunfan
Abstract excerpt
Currently, multiplex-PCR with genotype-specific primers is widely used for preliminary screening of hepatitis B virus (HBV) genotypes, despite its relatively lower accuracy compared with whole genome sequencing. Here, we present the discrepant results of HBV genotyping by PCR and full-length sequencing. HBV DNA was isolated from chronic hepatitis B serum and the HBV genotype was detected by PCR using...
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