Article
freqpcr: Estimation of population allele frequency using qPCR ΔΔCq measures from bulk samples.
Molecular ecology resources - 1 May 2022
Sudo Masaaki, Osakabe Masahiro
Abstract excerpt
PCR techniques, both quantitative (qPCR) and nonquantitative, have been used to estimate the frequency of a specific allele in a population. However, the labour required to sample numerous individuals and subsequently handle each sample renders the quantification of rare mutations (e.g., pesticide resistance gene mutations at the early stages of resistance development) challenging. Meanwhile, pooling DNA from...
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