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Clinical establishment of a laboratory developed quantitative HDV PCR assay on the cobas6800 high-throughput system

28 Aug 2021

Abstract excerpt

Background & Aims Currently available HDV PCR assays are characterized by considerable run-to-run and inter-laboratory variability. Hence, we established a quantitative reverse transcription real-time PCR (RT-qPCR) assay on the open channel of a fully automated PCR platform (cobas6800, Roche) offering improved consistency and reliability. Methods A primer/probe-set targeting a highly conserved region upstream of...

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PMID: 34712932
DOI: 10.1016/j.jhepr.2021.100356

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