Article
Fingerprint construction through genotyping by sequencing for applied breeding in Brassica rapa.
Genome - 1 Feb 2022
Li Guoliang, Yue Lixin, Cai Xu, Li Fei, Zhang Hui, Zhang Shujiang, Zhang Shifan, Sun Rifei
Abstract excerpt
This study evaluated the genotyping by sequencing (GBS) protocol for fingerprinting Brassica rapa, and the data derived were more reliable than the re-sequencing data of B. rapa. Of the 10 enzyme solutions used to analyze the numbers of genotypes and single-nucleotide polymorphisms (SNPs) in B. rapa, five solutions showed better results, namely, A (HaeIII, 450-500 bp), E (RsaI+HaeIII, 500-550 bp), F (RsaI+HaeIII,...
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