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Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

30 Sept 2021

Abstract excerpt

Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein were found to...

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PMID: 34592012
DOI: 10.1111/lam.13567

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