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Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification— SYBR green I assays

20 Aug 2018

Abstract excerpt

BACKGROUND: Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, SYBR Green I was used for endpoint detection of the RPA products by the naked eye. METHOD: A total of 146 genomic Mtb...

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PMID: 30129085
DOI: 10.1002/jcla.22655

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