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Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology.

Genetic testing and molecular biomarkers - 1 Sept 2021

Tyumentseva Anna V, Yaroslavtsev Roman N, Stolyar Sergey V, Saitova Alina T, Tyutrina Ekaterina S, Gorbenko Alexey S, Stolyar Marina A, Olkhovskiy Igor A

Abstract excerpt

Aim: To develop magnetic nanoparticles (MNPs) based on iron oxide for DNA isolation from blood cells for quantitative molecular genetic analyses of the V617F mutation in the Januskinase 2 (JAK2) gene. Materials and Methods: MNPs were synthesized by the coprecipitation method and coated with tetraethyl orthosilicate (TEOS). The size and shape of the complexes were estimated using transmission electron microscopy....

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PMID: 34550778
DOI: 10.1089/gtmb.2021.0067

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