Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.

doi:10.1016/j.ijid.2021.09.006

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Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Nov 2021

Hasan Mohammad R, Kalikiri Mahesh K R, Mirza Faheem, Sundararaju Sathyavathi, Sharma Anju, Xaba Thabisile, Lorenz Stephan, Chemaitelly Hiam, El-Kahlout Reham A, Tsui Kin Ming, Yassine Hadi M, Coyle Peter V, Al Khal Abdullatif, Bertollini Roberto, Al Thani Mohamed H, Abu-Raddad Laith J, Tang Patrick

Abstract excerpt

Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792...

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PMID: 34525398
DOI: 10.1016/j.ijid.2021.09.006

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