Article
Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2.
Virus genes - 1 Oct 2021
Zhang Lin, Jiang Wenming, Zhang Fuyou, Li Yang, Li Jinping, Liang Shaobo, Yu Xiaohui, Peng Cheng, Liu Shuo, Wang Jingjing, Sun Shuhong, Liu Hualei
Abstract excerpt
In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs)...
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