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Article

A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions.

Journal of virological methods - 1 Oct 2021

Chaintoutis Serafeim C, Chassalevris Taxiarchis, Tsiolas George, Balaska Sofia, Vlatakis Ioannis, Mouchtaropoulou Evangelia, Siarkou Victoria I, Tychala Areti, Koutsioulis Dimitris, Skoura Lemonia, Argiriou Anagnostis, Dovas Chrysostomos I

Abstract excerpt

The emergence of SARS-CoV-2 mutations resulting in the S protein amino-acid substitutions N501Y and E484K, which have been associated with enhanced transmissibility and immune escape, respectively, necessitates immediate actions, for which their rapid identification is crucial. For the simultaneous typing of both of these mutations of concern (MOCs), a one-step real-time RT-PCR assay employing four locked nucleic...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34274369
DOI: 10.1016/j.jviromet.2021.114242
PMCID: PMC8282437

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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