Article
High-throughput sequencing-based microsatellite genotyping for polyploids to resolve allele dosage uncertainty and improve analyses of genetic diversity, structure and differentiation: A case study of the hexaploid Camellia oleifera.
Molecular ecology resources - 1 Jan 2022
Cui Xiangyan, Li Caihua, Qin Shengyuan, Huang Zebin, Gan Bin, Jiang Zhengwen, Huang Xiaomao, Yang Xiaoqiang, Li Qin, Xiang Xiaoguo, Chen Jiakuan, Zhao Yao, Rong Jun
Abstract excerpt
Conventional microsatellite (simple sequence repeat, SSR) genotyping methods cannot accurately identify polyploid genotypes leading to allele dosage uncertainty, introducing biases in population genetic analysis. Here, a new SSR genotyping method was developed to directly infer accurate polyploid genotypes. The frequency distribution of SSR sequences was obtained based on deep-coverage high-throughput sequencing...
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