SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B.1.1.7 lineage in a commercial multiplex PCR assay.

doi:10.1016/j.cmi.2021.05.025

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SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B.1.1.7 lineage in a commercial multiplex PCR assay.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases - 1 Sept 2021

Wollschläger Paul, Todt Daniel, Gerlitz Nadja, Pfaender Stephanie, Bollinger Thomas, Sing Andreas, Dangel Alexandra, Ackermann Nickolaus, Korn Klaus, Ensser Armin, Steinmann Eike, Buhl Michael, Steinmann Joerg

Abstract excerpt

OBJECTIVES: Detection and surveillance of SARS-CoV-2 is of eminent importance, particularly due to the rapid emergence of variants of concern (VOCs). In this study we evaluated if a commercially available quantitative real-time PCR (qRT-PCR) assay can identify SARS-CoV-2 B.1.1.7 lineage samples by a specific N gene dropout or Ct value shift compared with the S or RdRp gene. METHODS: VOC B.1.1.7 and non-B.1.1.7...

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PMID: 34044153
DOI: 10.1016/j.cmi.2021.05.025
PMCID: PMC8142743

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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