Article
Biofilm viability checker: An open-source tool for automated biofilm viability analysis from confocal microscopy images.
NPJ biofilms and microbiomes - 14 May 2021
Mountcastle Sophie E, Vyas Nina, Villapun Victor M, Cox Sophie C, Jabbari Sara, Sammons Rachel L, Shelton Richard M, Walmsley A Damien, Kuehne Sarah A
Abstract excerpt
Quantifying biofilm formation on surfaces is challenging because traditional microbiological methods, such as total colony-forming units (CFUs), often rely on manual counting. These are laborious, resource intensive techniques, more susceptible to human error. Confocal laser scanning microscopy (CLSM) is a high-resolution technique that allows 3D visualisation of biofilm architecture. In combination with a...
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