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Article

Site-Directed Mutagenesis of Bifidobacterium Strains.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

James Kieran, van Sinderen Douwe

Abstract excerpt

At present, only a limited number of Bifidobacterium species are amenable to genetic manipulation using mutagenesis. This lack of genetic accessibility among the majority of bifidobacterial strains represents a significant roadblock for the study of gene function and expression in these potential probiotics. Genetic tools for generating mutants are difficult to develop for bifidobacteria, as they require...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 33649947
DOI: 10.1007/978-1-0716-1274-3_5

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