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Article

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator.

Journal of visualized experiments : JoVE - 9 Feb 2021

Sieber Kayli, Saar Maya, Opachaloemphan Comzit, Gallitto Matthew, Yang Huan, Yan Hua

Abstract excerpt

The unique traits of eusocial insects, such as social behavior and reproductive division of labor, are controlled by their genetic system. To address how genes regulate social traits, we have developed mutant ants via delivery of CRISPR complex into young embryos during their syncytial stage. Here, we provide a protocol of CRISPR-mediated mutagenesis in Harpegnathos saltator, a ponerine ant species that displays...

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Identifiers

PMID: 33645561
DOI: 10.3791/61930

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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