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Article

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis.

Journal of visualized experiments : JoVE - 4 Dec 2020

Dalla Benetta Elena, Chaverra-Rodriguez Duverney, Rasgon Jason L, Akbari Omar S

Abstract excerpt

The jewel wasp, Nasonia vitripennis, has become an efficient model system to study epigenetics of haplo-diploid sex determination, B-chromosome biology, host-symbiont interactions, speciation, and venom synthesis. Despite the availability of several molecular tools, including CRISPR/Cas9, functional genetic studies are still limited in this organism. The major limitation of applying CRISPR/Cas9 technology in N....

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PMID: 33346197
DOI: 10.3791/61892

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