Article
Establishment of a cell line from swim bladder of the Grass carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II.
Microbial pathogenesis - 1 Feb 2021
Yang Yuru, Wang Yingying, Wang Qing, Zeng Weiwei, Li Yingying, Yin Jiyuan, Wu Siyu, Shi Cunbin
Abstract excerpt
A cell line was established from swim bladder of the Grass carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 °C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at...
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