Article
A novel method for isolation and culture of primary swine gastric epithelial cells.
BMC molecular and cell biology - 6 Jan 2021
Bautista-Amorocho Henry, Silva-Sayago Jorge Alexander, Goyeneche-Patino Diego A, Pérez-Cala Tania Liseth, Macías-Gómez Fabio, Arango-Viana Juan Carlos, Martínez Alonso
Abstract excerpt
BACKGROUND: Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a surrogate model for human diseases. Several porcine cell lines have been established based on a variety of tissues, which have shown to extensively...
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