Article
TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.
Nature communications - 22 Dec 2020
Yu Changwei, Cvetesic Nevena, Hisler Vincent, Gupta Kapil, Ye Tao, Gazdag Emese, Negroni Luc, Hajkova Petra, Berger Imre, Lenhard Boris, Müller Ferenc, Vincent Stéphane D, Tora László
Abstract excerpt
During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses...
Topics
- Animals
- Animals, Newborn
- Female
- Gene Expression Regulation
- Mice
- Mice, Inbred C57BL
- Mutation
- NIH 3T3 Cells
- Nuclear Proteins
- Oocytes
- Promoter Regions, Genetic
- RNA, Messenger
- TATA Box