Article
Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni.
BMC genetics - 18 Dec 2020
Choo Amanda, Fung Elisabeth, Chen Isabel Y, Saint Robert, Crisp Peter, Baxter Simon W
Abstract excerpt
BACKGROUND: Pest eradication using the Sterile Insect Technique (SIT) involves high-density releases of sterilized males that mate with wild females and ultimately suppress the population. Sterilized females are not required for SIT and their removal or separation from males prior to release remains challenging. In order to develop genetic sexing strains (GSS), conditional traits such as temperature sensitive...
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