Article
Alternate PCR assays for screening of JH1 mutation associated with embryonic death in Jersey cattle.
Molecular and cellular probes - 1 Feb 2021
Kumar Anshuman, Gupta Ishwar Dayal, Mohan Govind, M R Vineeth, D Ravi Kumar, S Jayakumar, Kataria Ranjit Singh, Niranjan Saket Kumar
Abstract excerpt
Jersey haplotype (JH) 1, a stop-gain lethal mutation in the CWC15 gene, causes embryonic losses in Jersey cattle. Two PCR based assays using Amplification Refractory Mutation System (T-ARMS-PCR) and restriction fragment length polymorphism (PCR-RFLP) were developed for screening of the JH1 in cattle. During the screening, seven among 30 Indian Jersey bulls were identified as carriers of the mutant JH1 allele, the...
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