Article
Comparison of different hypervariable regions of 16S rRNA for taxonomic profiling of vaginal microbiota using next-generation sequencing.
Archives of microbiology - 1 Apr 2021
Sirichoat Auttawit, Sankuntaw Nipaporn, Engchanil Chulapan, Buppasiri Pranom, Faksri Kiatichai, Namwat Wises, Chantratita Wasun, Lulitanond Viraphong
Abstract excerpt
The exploration of vaginal microbiota by using next-generation sequencing (NGS) of 16S ribosomal RNA (rRNA) gene is widely used. Up to now, different hypervariable regions have been selected to study vaginal microbiota by NGS and there is no standard method for analysis. The study aimed to characterize vaginal microbiota from clinical samples using NGS targeting the 16S rRNA gene and to determine the performance...
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