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Evaluation of a custom GeneRead™ massively parallel sequencing assay with 210 ancestry informative SNPs using the Ion S5™ and MiSeq platforms.

Forensic science international. Genetics - 1 Jan 2021

Truelsen Ditte, Pereira Vania, Phillips Chris, Morling Niels, Børsting Claus

Abstract excerpt

A custom GeneRead DNAseq SNP panel with 210 markers was evaluated using the Ion S5 and MiSeq sequencing platforms. Sensitivity, PCR cycle number, and the use of half volume of reagents for target enrichment and library preparation were tested. Furthermore, genotype concordance between results obtained with the different sequencing platforms and with known profiles generated using other sequencing assays was...

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PMID: 33176271
DOI: 10.1016/j.fsigen.2020.102411

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