Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.).

doi:10.1007/s11103-018-0749-2

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Article

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.).

Plant molecular biology - 1 Jul 2018

Singh Manjit, Kumar Manish, Albertsen Marc C, Young Joshua K, Cigan A Mark

Abstract excerpt

KEY MESSAGE: Hexaploid bread wheat is not readily amenable to traditional mutagenesis approaches. In this study, we show efficient utilization of CRISPR-Cas system and Next Generation Sequencing for mutant analysis in wheat. Identification and manipulation of male fertility genes in hexaploid bread wheat is important for understanding the molecular basis of pollen development and to obtain novel sources of...

Topics

Amino Acid Sequence
CRISPR-Cas Systems
Flowers
Mutation
Plant Infertility
Plant Proteins
Pollen
Sequence Alignment
Triticum

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Open in PubMed

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PMID: 29959585
DOI: 10.1007/s11103-018-0749-2

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