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Derivation and propagation of spermatogonial stem cells from human pluripotent cells

23 Sept 2020

Abstract excerpt

Abstract Objectives This study is designed to generate and propagate human spermatogonial stem cells (SSCs) derived from human pluripotent stem cells (hPSCs). Methods hPSCs were differentiated into SSC-like cells (SSCLCs) by a three-step strategy. The biological characteristics of SSCLCs were detected by immunostaining with antibodies against SSC markers. The ability of self-renewal was measured by propagating...

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PMID: 32967715
DOI: 10.1186/s13287-020-01896-0

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