Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis.

doi:10.1007/978-1-0716-0939-2_8

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Article

Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Hasegawa Yoshiaki, Nagano Keiji, Murakami Yukitaka, Lamont Richard J

Abstract excerpt

Fimbriae of the periodontal pathogen Porphyromonas gingivalis mediate its colonization through associations with other bacteria and host tissues. P. gingivalis generally expresses two distinct fimbrial types, FimA and Mfa1. In P. gingivalis ATCC 33277, FimA fimbriae are present as long filaments easily detached from cells, whereas Mfa1 fimbriae are short filaments compactly bound to the cell surface. Because of...

Topics

Bacterial Adhesion
Bacterial Proteins
Electrophoresis, Polyacrylamide Gel
Fimbriae Proteins
Fimbriae, Bacterial
Humans
Immunoblotting
Mutation
Porphyromonas gingivalis

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Open in PubMed

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PMID: 32815129
DOI: 10.1007/978-1-0716-0939-2_8

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