Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

International journal of molecular sciences - 4 Aug 2020

Gand Mathieu, Vanneste Kevin, Thomas Isabelle, Van Gucht Steven, Capron Arnaud, Herman Philippe, Roosens Nancy H C, De Keersmaecker Sigrid C J

Abstract excerpt

The current COronaVIrus Disease 2019 (COVID-19) pandemic started in December 2019. COVID-19 cases are confirmed by the detection of SARS-CoV-2 RNA in biological samples by RT-qPCR. However, limited numbers of SARS-CoV-2 genomes were available when the first RT-qPCR methods were developed in January 2020 for initial in silico specificity evaluation and to verify whether the targeted loci are highly conserved. Now...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Betacoronavirus
Share to Coronavirus Infections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to COVID-19

Share to Pandemics

Share to Pneumonia, Viral

Share to RNA-Dependent RNA Polymerase

Share to SARS-CoV-2

Share to Whole Genome Sequencing

Source

Open in PubMed Open full text

Identifiers

PMID: 32759818
DOI: 10.3390/ijms21155585
PMCID: PMC7432934

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.PMID 32759818Read ↗

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants.Semantic proximity Read ↗

Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological AdvantageSemantic proximity Read ↗

Evaluation of a Rapid and Accessible RT-qPCR Approach for SARS-CoV-2 Variant of Concern IdentificationSemantic proximity Read ↗

Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification.Semantic proximity Read ↗

Implementation of an In-House Platform for Rapid Screening of SARS-CoV-2 Genome Variations.Semantic proximity Read ↗

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequencesSemantic proximity Read ↗

Molecular biology of SARS-CoV-2 and techniques of diagnosis and surveillance.Semantic proximity Read ↗

A Method to evaluate analytical sensitivity of qRT-PCR kits for SARS-CoV-2 detectionSemantic proximity Read ↗

Evaluation of Analytical and Clinical Performance and Usefulness in a Real-Life Hospital Setting of Two in-House Real-Time RT-PCR Assays to Track SARS-CoV-2 Variants of Concern.Semantic proximity Read ↗

Design of a Novel Multiplex Real Time RT-PCR Assay for SARS-CoV-2 DetectionSemantic proximity Read ↗

Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2Semantic proximity Read ↗

Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrixSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.