Article
Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Erban Alexander, Martinez-Seidel Federico, Rajarathinam Yogeswari, Dethloff Frederik, Orf Isabel, Fehrle Ines, Alpers Jessica, Beine-Golovchuk Olga, Kopka Joachim
Abstract excerpt
This book chapter describes the analytical procedures required for the profiling of a metabolite fraction enriched for primary metabolites. The profiling is based on routine gas chromatography coupled to mass spectrometry (GC-MS). The generic profiling method is adapted to plant material, specifically to the analysis of plant material that was exposed to temperature stress. The method can be combined with stable...
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