Article
Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.
Plant physiology - 1 Dec 2005
Bölling Christian, Fiehn Oliver
Abstract excerpt
A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight...
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